cytometer Options
cytometer Options
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Information-enriched characterization of cell populations. Morphological information from photos can insert for the richness of flow cytometry knowledge. As an example, the figure displays an usually standard apoptosis assay employing Annexin V and PI, adding mobile imaging to characterize cells in Every single inhabitants to expose morphologically distinctive functions. These insights could not have already been acquired from multiplex staining by yourself.
As samples are obtained about the Attune CytPix Flow Cytometer, the superior-speed brightfield digicam captures and stores photos of detected occasions in a level up to six,000 pictures for each next, with regards to the flow amount and graphic dimension. For higher versatility, Attune Cytometric Software package allows you to modify the picture seize frequency as needed and select a specific gate for impression seize, if expected.
Combine acoustic concentrating know-how with large-parameter Investigation for versatile spectral unmixing and traditional workflows.
Find out new insights into your experimental knowledge by visualizing populations working with merged fluorescence and morphology information. Our program interface wizards allow downsampling, concatenation (merged data files) and dimensionality tools which are constructed in the software package for an intuitive, streamlined data Assessment expertise.
"An image just isn't quite well worth a thousand dots, but it surely really helps to see illustrations or photos. It is great to find out photos that correlate with staining. By way of example, AnnexinV-positive cells which can be morphologically unique than balanced cells, or CD14+ monocytes which might be much larger and more textured than CD3+/CD4+/CD14- lymphocytes.
Also, it truly is illuminating to view how many doublets slip through traditional singlet gates, and fantastic to verify the status of surprising double-beneficial activities in my Evaluation."
Optimizing gating system. Even sturdy guide singlet gating is error-vulnerable and continues to be a subjective choice position in Virtually all flow cytometry assays. Imaging can be employed to substantiate and change gates to include only one cells of fascination.
Consistent picture top quality even at significant throughput Acoustic concentrating along with a large-speed digicam Merge to picture these T cells continually at small or superior flow prices. Easily regulate focus and digital camera configurations to meet experimental needs.
Early signs of this contamination had at first been dismissed as particles. Backgating to find new populations. Backgating imaged cells over the Attune CytPix Flow Cytometer also lets you use morphological capabilities to find out fascinating subpopulations that might not be obvious from flow cytometry knowledge by yourself.
Perhaps most significantly, these situations comprise cells of Obviously distinctive phenotypes which could lead to incorrect conclusions with regards to https://www.mazafakas.com/user/profile/6210340 double beneficial events (particularly in unusual populations).
AI-pushed automated graphic Examination Together with the Attune Cytometric Software program translates function characteristics into distinct morphology parameters that could be combined with common fluorescence and scatter parameters to reveal extremely reproducible populace adjustments and one of a kind subsets.
Software Observe: Automatic image Examination cuts down consumer-to-consumer variability in flow cytometry gating procedures
Built-in dimensionality reduction equipment greater resolution into populations. Fluorescently gated populations back again-gated on to UMAP plot to confirm predicted inhabitants resolution in lysed total human blood. Person can look at gatherings from Each and every cluster for visual affirmation of cell phenotypes.
Flexible payment strategy from Agilent Monetary Alternatives allow you to get the significant instrumentation you will need for fast venture, or to increase your lab.
Gating on these prolonged parameters lets you promptly and properly identify populations of fascination and confirm gating method with little if any handbook assessment. The Attune software presents usage of point out-of-the-artwork machine Finding out Examination equipment within an expertly made person interface, rendering it extremely simple to operate.
Since the cell line experienced Formerly been analyzed within the Attune CytPix Flow Cytometer, the researchers went back to the photographs and have been in a position to doc the microbial infection no less than 5 days before.
Agilent Worth Guarantee ensures you a minimum of 10 years of instrument use in the day you purchase. Normally, We are going to credit history you with the residual price of the program toward an upgraded model.
Right here, we present that by gating approaches employing impression-primarily based quantitative parameters can distinguish interacting cells from coincident functions to far more accurately analyze interacting cells.